Pancreatic Tissue Immunohistochemistry

Following fixation in 10% NBF for 24–48 h, pancreatic tissue was embedded in paraffin, sectioned, stained, and analyzed as previously described [20 (link),21 (link)]. Primary antibodies used were as follows: guinea pig anti-insulin (Invitrogen, Grand Island, NY, USA; #18-0067; 1:800), glucagon (Cell Signaling Technology, Danvers, MA, USA; #2760; 1:300), Nkx6.1 (Developmental Studies Hybridoma Bank, Iowa City, IA, USA; #F55A12; 1:100); and Aldh1a3 (Novus Biologicals, Centennial, CO, USA; #NBP2-15339; 1:100).

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Collier J.J., Batdorf H.M., Merrifield K.L., Martin T.M., White U., Ravussin E., Burk D.H., Cooley C.R., Karlstad M.D, & Burke S.J. (2021). Pioglitazone Reverses Markers of Islet Beta-Cell De-Differentiation in db/db Mice While Modulating Expression of Genes Controlling Inflammation and Browning in White Adipose Tissue from Insulin-Resistant Mice and Humans. Biomedicines, 9(9), 1189.

Publication 2021

guinea pig anti-insulin glucagon Aldh1a3

Antibodies Biologicals Embedded paraffin Glucagon Glucagon cell Guinea pig Hybridoma Insulin Novus Pancreatic Tissue

Corresponding Organization : Pennington Biomedical Research Center

Other organizations : University of Tennessee at Knoxville, University of Tennessee Health Science Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of insulin, glucagon, Nkx6.1, and Aldh1a3 in pancreatic tissue

control variables

Fixation time in 10% NBF (24-48 h)
Embedding in paraffin
Sectioning
Staining procedure

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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