ChIP Assays for DREAM, MMB, and E2F Complexes

ChIP assays were described earlier [23 (link), 43 (link)]. The following antibodies were used to precipitate DREAM, MMB, and E2F complex components: E2F4 (C-20, sc-866; Santa Cruz Biotech.) [44 (link)], E2F1 (C-20, sc-193; Santa Cruz Biotech.), E2F2 (C-20, sc-633; Santa Cruz Biotech.), E2F3 (C-18, sc-878; Santa Cruz Biotech.), LIN37 (T1, custom-made at Pineda Antikörper-Service, Berlin, Germany), BMYB (N-19, Santa Cruz Biotech.), FOXM1 (C-20, sc-502, Santa Cruz Biotech.) [33 (link)], and NF-YA (G-2, sc-17753; Santa Cruz Biotech.). The E2F3 antibody was directed against the C terminus of the protein and did not distinguish between E2F3A and E2F3B, which differ in their N-terminal domain [45 (link)]. As a negative control for the ChIP experiments, rabbit IgG was used (Rabbit IgG Isotype control, No. 02-6102; ThermoFisher Scientific).

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Uxa S., Castillo-Binder P., Kohler R., Stangner K., Müller G.A, & Engeland K. (2021). Ki-67 gene expression. Cell Death and Differentiation, 28(12), 3357-3370.

Publication 2021

LIN37

Antibodies Antibody Chip Chip assays Dream E2f1 Isotype Protein c Rabbit

Corresponding Organization :

Other organizations : Leipzig University

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Variable analysis

independent variables

Antibodies used to precipitate DREAM, MMB, and E2F complex components: E2F4, E2F1, E2F2, E2F3, LIN37, BMYB, FOXM1, and NF-YA

dependent variables

Not explicitly mentioned

control variables

Rabbit IgG used as a negative control for ChIP experiments

controls

None specified
Rabbit IgG Isotype control

Annotations

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