Samples (n = 61) were processed for both total RNA, as well as miRNA extraction. In brief, total RNA and miRNAs were extracted using the Trizol standard protocol (Invitrogen, Carlsbad, CA, USA) and the mirVANA miRNA isolation kit (Ambion, Austin, TX, USA). The RNA quantity and quality were evaluated using a spectrophotometer (NanoDrop® ND-1000 UV–vis, Nanogen Inc., San Diego, CA, USA), as previously described [3 (link),4 (link),5 (link)].
Free full text: Click here