3D constructs were fabricated as previously described.15 (link) In summary, monomeric
collagen type I of rat tail origin (First Link, Birmingham, UK) was mixed with
10X MEM (Gibco™ through Thermo Fisher Scientific, Loughborough, UK) and
neutralising agent (N.A.) according to the RAFT™ protocol. After cellular
addition, cross-linking is performed by adding 240 µL or 1.3 mL of cellular
collagen mix into a 96-well or 24-well plate respectively, followed by
incubation at 37°C for 15 min. Plastic compression is performed using RAFT™
absorbers (Lonza, Slough, UK) for 15 min resulting in dense 10% collagen
constructs.14 (link) In order to adhere different layers of dense collagen
gels producing multicellular constructs as described inFigure 1 and Table 1 , a drop of collagen mix was
used.
collagen type I of rat tail origin (First Link, Birmingham, UK) was mixed with
10X MEM (Gibco™ through Thermo Fisher Scientific, Loughborough, UK) and
neutralising agent (N.A.) according to the RAFT™ protocol. After cellular
addition, cross-linking is performed by adding 240 µL or 1.3 mL of cellular
collagen mix into a 96-well or 24-well plate respectively, followed by
incubation at 37°C for 15 min. Plastic compression is performed using RAFT™
absorbers (Lonza, Slough, UK) for 15 min resulting in dense 10% collagen
constructs.14 (link) In order to adhere different layers of dense collagen
gels producing multicellular constructs as described in
used.
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