T. gondii tachyzoites ME49 (clone B7–21), ME49-GFP-Luc1 and Pru-Δhxgprt-tdTomato (Pru-tdTomato)2 were propagated in confluent human foreskin fibroblasts (HFF1) in DMEM medium containing high glucose supplemented with 100 U ml−1 penicillin, 100 mg ml−1 streptomycin and 2 % fetal bovine serum.
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Culturing T. gondii Tachyzoites in HFF Cells
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Corresponding Organization : University Medical Center Freiburg
Other organizations : Humboldt-Universität zu Berlin, Charité - Universitätsmedizin Berlin, Freie Universität Berlin, The Francis Crick Institute, Robert Koch Institute, Durham VA Health Care System, Geriatric Research Education and Clinical Center, Duke University, Duke University Hospital, Duke Medical Center
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Variable analysis
independent variables
- T. gondii tachyzoites ME49 (clone B7–21)
- ME49-GFP-Luc
- Pru-Δhxgprt-tdTomato (Pru-tdTomato)
- Not explicitly mentioned
- Human foreskin fibroblasts (HFF1)
- DMEM medium containing high glucose
- 100 U ml^-1 penicillin
- 100 mg ml^-1 streptomycin
- 2% fetal bovine serum
- No positive or negative controls specified
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