The number of MFAPs and the MFCV values was estimated to be 20 per subject.
The MFAP has confirmed after 50–100 consecutive discharges a time variability (MCD) of < 5 μs, similar to the number used for the neuromuscular jitter (6 (link)). However, < 50 could be acceptable to confirm a direct muscle fiber stimulation.
The mean MFCV value, the fastest divided by the slowest MFCV from each subject (F/S ratio), and the semi-quantitative MUAP amplitude values obtained by needle EMG were one value per subject. An example of the F/S ratio calculation is shown in
Depending on the normality test, some variables have mean or median values.