Amniotic Fluid and Blood Collection for Research

20-25 mL of AF and 3-5 mL of blood were obtained from the pregnant women at the time of amniocentesis. The AF was centrifuged at 1200 rpm for 10 min at 4°C, and the supernatant was collected. Blood was placed at 4°C for 1 h and centrifuged at 3000 rpm at 4°C for 10 min, and serum was collected from the upper layer. All samples were stored at -80°C before analysis, and their use for research was approved by the ethical committee. In the validation cohort, AF and blood samples were obtained from the same pregnant woman.

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Yuan H., Liu C., Wang X., Huang T., Liu D., Huang S., Wu Z., Liu Y., Yin P, & Yang B. (2023). Association between aberrant amino acid metabolism and nonchromosomal modifications fetal structural anomalies: A cohort study. Frontiers in Endocrinology, 14, 1072461.

Publication 2023

Amniocentesis Blood Pregnant woman Serum

Corresponding Organization :

Other organizations : Jiangxi Maternal and Child Health Hospital, Dalian Medical University, First Affiliated Hospital of Dalian Medical University, Dalian Institute of Chemical Physics, Chinese Academy of Sciences

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Variable analysis

independent variables

Amount of amniotic fluid (AF) obtained (20-25 mL)
Amount of blood obtained (3-5 mL)

dependent variables

Not explicitly mentioned

control variables

Centrifugation of AF at 1200 rpm for 10 min at 4°C
Centrifugation of blood at 3000 rpm for 10 min at 4°C
Storage of all samples at -80°C before analysis
Use of samples for research approved by ethical committee
Samples obtained from the same pregnant woman in the validation cohort

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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