Primary rat neural progenitor cells isolated from the hippocampi of 6-week-old female Fisher 344 rats (Charles River) were cultured in growth medium (DMEM/F12 (Life Technologies) containing N2 supplement (Life Technologies) and 10 ng/mL FGF-2 (PeproTech)) on laminin (Roche) and polyornithine (Sigma) coated tissue culture plates, with subculturing on reaching 80% confluency using Accutase (Phoenix Flow Systems), as previously described [54 (link)].
Primary mouse neural progenitor cells were isolated form C57BL6/J mice (Charles River) as previously described [56 (link)]. Cells were cultures in growth medium (Neurobasal A (Gibco) containing B27 supplement (Gibco), Glutamax-1 supplement (Gibco), 20 ng/mL FGF-2 (Peprotech), and 20 ng/mL EGF (PeproTech)) on Poly-d-Lysine (Sigma) and laminin (Roche) coated tissue culture plate, with subculturing on reaching 80% confluency using Accutase (Phoenix Flow Systems). Progenitor cells were tested for mycoplasma contamination at the UC Berkeley Stem Cell Core Facility and using Hoechst DNA stain.
Primary mouse neural progenitor cells were isolated form C57BL6/J mice (Charles River) as previously described [56 (link)]. Cells were cultures in growth medium (Neurobasal A (Gibco) containing B27 supplement (Gibco), Glutamax-1 supplement (Gibco), 20 ng/mL FGF-2 (Peprotech), and 20 ng/mL EGF (PeproTech)) on Poly-d-Lysine (Sigma) and laminin (Roche) coated tissue culture plate, with subculturing on reaching 80% confluency using Accutase (Phoenix Flow Systems). Progenitor cells were tested for mycoplasma contamination at the UC Berkeley Stem Cell Core Facility and using Hoechst DNA stain.
