C28/I2 WT chondrocytes [53 (link)] were cultured at 37 °C, 5% CO2, and 100% humidity in DMEM (Biochrom, Berlin, Germany) supplemented with 10% FCS, 1% sodium pyruvate, 100 units/mL penicillin, and 100 μg/mL streptomycin (DMEM complete). ERp57 knockout C28/I2 cells (C28/I2 KO) were generated using CRISPR/Cas9 as previously described [50 (link)] and cultured likewise.
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