Targeted LC-MS/MS Analysis of Compounds

HPLC separation was accomplished using a (Waters) reversed-phase X select HSS T3 column (2.1×150 mm, 2.5 µm), a (Phenomenex) precolumn, and in-Line filter disks (0.5 µm × 3.0 mm). Adopting conditions described by [17 (link)]. PeakView^TM software was used to compare retention time and m/z values obtained by MS and MS² to identify compounds. PeakView^TM software’s XIC Manager was used to calculate peak area values. For each targeted analyte, extracted ion chromatograms (XICs) were automatically generated and compared to a user-defined threshold [51 (link)]. The LC-MS/MS analysis was carried out in Proteomics and Metabolomics Unit, Children’s Cancer Hospital (57357).

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Elmongy E.I., Negm W.A., Elekhnawy E., El-Masry T.A., Attallah N.G., Altwaijry N., Batiha G.E, & El-Sherbeni S.A. (2022). Antidiarrheal and Antibacterial Activities of Monterey Cypress Phytochemicals: In Vivo and In Vitro Approach. Molecules, 27(2), 346.

Publication 2022

reversed-phase X select HSS T3 column precolumn

Children Hplc Line Ms ms Retention

Corresponding Organization : Tanta University

Other organizations : Princess Nourah bint Abdulrahman University, Helwan University, Damanhour University

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Variable analysis

independent variables

The HPLC separation conditions (column, precolumn, in-Line filter disks) described by [17 (link)]

dependent variables

Retention time
M/z values obtained by MS and MS^2
Peak area values

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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