Immunohistochemical Profiling of Tumor Samples

Collected tumours or normal tissue after removal of paraffin as the experimental subject. Consecutive 4-μm thick unstained sections for immunohistochemical staining, which was performed using the Leica automatic immunostaining device (Leica Microsystems, Inc.). Primary antibodies against CD10 (1:100; no. 563871; DAKO; DK), α-inhibin (1:100; no. GT230202; CHN), SMA (1:50; no. MAB-0980; MXB; CHN), desmin (1:300; no. GT225202; Gene tech; CHN), TFE3 (1:100; no. ZA-0657; ZSGB-BIO; CHN), calretinin (1:100; no. ZM-0063; ZSGB-BIO; CHN), WT-1 (1:100; no. ZM-0269; ZSGB-BIO; CHN), and EMA (1:300; no. GM061302; Gene tech; CHN). Appropriate positive and negative controls were simultaneously stained to validate the staining method.