Five Petri dishes and eight whole zygotic embryos per Petri dish, cytokinin type, and light treatment were cultured in HLP. When the axillary shoots were isolated and cultured individually in the elongation medium, the EFS, the NS/E, and percentage of (PSR) out of the total number of shoots produced per explant were calculated. A logistic regression model was used to analyze the effect of the cytokinin type and light treatment on survival and EFS. When necessary, Tukey’s post hoc test (α = 0.05) was used for multiple comparisons.
The confirmation of the homogeneity of variances and normality of the data on the NS/E and PSR were performed, and PSR was transformed to meet homocedasticity. Data for the NS/E and PSR were analyzed by analysis of variance (ANOVA). When necessary, multiple comparisons were made using Tukey’s post hoc test (α = 0.05).
A completely randomized design was carried out using six to twenty plantlets per cytokinin type and light treatment per each auxin treatment. The RI percentage (RI), the mean NR/E, and the LLR (cm) were recorded after six weeks of culture in a root elongation medium.
To assess the effect of the cytokinin and light treatment on the RI, a logistic regression was performed. Data for NR/E and LLR were analyzed by ANOVA. When necessary, multiple comparisons were made using Tukey’s post hoc test (α = 0.05). The acclimatization percentage was calculated after four weeks under ex vitro conditions. The data were analyzed using R Core Team software® (version 4.2.1, Vienna, Austria).
The confirmation of the homogeneity of variances and normality of the data on the NS/E and PSR were performed, and PSR was transformed to meet homocedasticity. Data for the NS/E and PSR were analyzed by analysis of variance (ANOVA). When necessary, multiple comparisons were made using Tukey’s post hoc test (α = 0.05).
A completely randomized design was carried out using six to twenty plantlets per cytokinin type and light treatment per each auxin treatment. The RI percentage (RI), the mean NR/E, and the LLR (cm) were recorded after six weeks of culture in a root elongation medium.
To assess the effect of the cytokinin and light treatment on the RI, a logistic regression was performed. Data for NR/E and LLR were analyzed by ANOVA. When necessary, multiple comparisons were made using Tukey’s post hoc test (α = 0.05). The acclimatization percentage was calculated after four weeks under ex vitro conditions. The data were analyzed using R Core Team software® (version 4.2.1, Vienna, Austria).
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