All isolates were identified as members of the M. tuberculosis complex, using gene probes (ACCUProbe; Hologic, San Diego, CA, USA) or the GenoType MTBC assay (Hain Lifescience, Nehren, Germany). The differentiation between the species of the M. tuberculosis complex was based on genotyping data of each individual isolate [7 (link)–9 (link)]. Drug susceptibility testing was determined by means of the proportioning method on Löwenstein–Jensen medium and/or by means of the modified proportioning method with the BACTEC MGIT 960 system (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA).
Protocol detail
M. tuberculosis Complex Identification
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Assay
Gene
M tuberculosis
Susceptibility
Corresponding Organization :
Other organizations : University Hospital Schleswig-Holstein, University of Lübeck, German Center for Lung Research, German Center for Infection Research, Research Center Borstel - Leibniz Lung Center, Berufsgenossenschaft für Gesundheitsdienst und Wohlfahrtspflege, University Medical Center Hamburg-Eppendorf, Universität Hamburg
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Variable analysis
independent variables
- None explicitly mentioned
- Identification of isolates as members of the M. tuberculosis complex
- Differentiation between species of the M. tuberculosis complex
- Drug susceptibility of isolates
- None explicitly mentioned
- Positive control: Gene probes (ACCUProbe) or the GenoType MTBC assay to identify isolates as members of the M. tuberculosis complex
- Negative control: Not specified
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