Immune cells were isolated from mouse liver, as described previously.1 (link),27 (link) Immune cells were stained with CD45-APC-Cy7 (Becton Dickinson, Franklin Lakes, NJ), CD11b-PE (Becton Dickinson), F4/80-FITC (Miltenyi Biotec), and Ly6C-Pacific blue (MACS). Flow cytometry was carried out using BD LSR-Fortessa. Analysis was performed using FlowJo software (FlowJo 10.8.1, Ashland, OR).
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