A binge dosing regimen was used to induce METH neurotoxicity (Howard et al. 2011 (link); Howard et al. 2013a (link); Howard et al. 2013b (link)). This regimen reliably mimics aspects of the long-term neurotoxic effects of METH observed in humans, including loss of markers for DA and serotonin neurons and microglia activation (Marshall and O’Dell 2012 (link)). Briefly, animals were placed in plastic housing tubs (50 cm length × 40 cm width × 20 cm height; 4 rats/tub). Saline (0.9 %) or (±)-METH•HCl (7.5 mg/kg; dissolved in 0.9% saline; calculated as free base) was injected s.c. every two hours until a total of four injections were administered. Core body temperature was monitored rectally (Thermalert TH-5 thermometer, Physitemp, Clifton, NJ, USA) every hour during injections and for two additional hours after injections.
Protocol detail
Binge Dosing Regimen for METH Neurotoxicity
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0 9 saline
Animals
Body temperature
Humans
Long term effects
Meth
Microglia
Neurons
Neurotoxic
Rats
Regimen
Saline
Serotonin
Thermometer
Corresponding Organization :
Other organizations : Illinois State University, University of Utah
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Variable analysis
independent variables
- Administration of saline (0.9%) or (±)-METH•HCl (7.5 mg/kg; dissolved in 0.9% saline; calculated as free base)
- Loss of markers for DA and serotonin neurons
- Microglia activation
- Ambient conditions (plastic housing tubs of specified dimensions, 4 rats per tub)
- Core body temperature monitoring (using Thermalert TH-5 thermometer)
- Positive control: Administration of (±)-METH•HCl
- Negative control: Administration of saline (0.9%)
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