All chemicals were purchased from Fisher Scientific or Aldrich unless noted otherwise. Peptide Calibration Standard II for MALDI-TOF MS was purchased from Bruker. mNisA, NisC, HalA2 and HalM2 were produced in Escherichia coli as previously described.13 (link),20a (link) Haloduracin β was purified from Bacillus halodurans C-125, as described previously.26 (link)
Protocol detail
Purification and Characterization of Lantibiotics
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Corresponding Organization :
Other organizations : University of Illinois Urbana-Champaign, Howard Hughes Medical Institute
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Variable analysis
independent variables
- MNisA
- HalA2
- HalM2
- Haloduracin β
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Peptide Calibration Standard II for MALDI-TOF MS
- Negative control: None mentioned
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