The total RNA was isolated from frozen tissue samples and cultured hepatocytes. RNA isolation and real-time RT-PCR were conducted as previously described [35] . The mRNA levels were analyzed for SREBP1c, carbohydrate responsive element-binding protein (ChREBP), ACC1, FAS, CPT1a, VLDLr, glucokinase, glucose-6-phosphatase, TNFα, and/or IL-6.
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