Brazilin (CAS no. 474-07-7; purity ≥98%) and chelerythrine (CAS no. 34316-15-9; purity ≥98%) were purchased from Wuhan ChemFaces Biochemical Co., Ltd. (Wuhan, China). Brazilin and chelerythrine were dissolved in dimethyl sulphoxide (DMSO) (Sigma-Aldrich; Merck KGaA), and further diluted with culture medium until DMSO concentration was <0.1%. Five different drug concentrations of Brazilin (2.5, 5, 10, 25 and 50 µM) were given for 1 h prior to hypoxia stimulation. Cells were also pretreated with 1 µM chelerythrine for 1 h before Brazilin treatment in order to repress PKC activation, as described previously (22 (link)).
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Brazilin and Chelerythrine Cytoprotection
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Corresponding Organization :
Other organizations : China Medical University, First Hospital of China Medical University
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Variable analysis
independent variables
- Concentration of brazilin (2.5, 5, 10, 25 and 50 µM)
- Pretreatment with 1 µM chelerythrine to repress PKC activation
- Unspecified, likely related to the response to hypoxia stimulation
- DMSO concentration (<0.1%)
- Exposure time to brazilin and chelerythrine (1 h)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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