Isolation and Culture of Mouse Bone Marrow-Derived Dendritic Cells

Six- to eight-week-old female C57BL/6 and OT-I transgenic mice were purchased from Koatech and Jackson Laboratory, respectively. Three- to eight-month-old ferrets were purchased from Marshall BioResources. All animal experiments were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of the Korea Research Institute of Bioscience and Biotechnology (KRIBB) and were performed according to the Guidelines for Animal Experiments of the KRIBB. Animals were housed in a specific pathogen-free facility in the KRIBB. Bone marrow-derived DCs were generated as previously described18 (link). Mouse EL4 cells were purchased from the American Type Culture Collection. All cells were maintained in RPMI 1640 containing 10% heat-inactivated FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin (Gibco).