Telomere length was assessed on TMA sections containing areas of adenocarcinoma using a telomere-specific fluorescence in situ hybridization (FISH) probe and 4’,6-diamidino-2-phenylindole (DAPI) for labeling total nuclear DNA as previously described (6 (link),14 (link)). Each individual TMA spot on each TMA slide was imaged using the TissueFAXS Plus (Tissue Gnostics) automated microscopy workstation and Zeiss Z2 Axioimager microscope. The digitized fluorescent telomere FISH signals were quantified using the TissueQuest software module to analyze the fluorescent images with precise nuclear segmentation. For each case, we evaluated prostate cancer cells and prostate CAS cells. Based on their unique morphologic features, other cell types (e.g. infiltrating lymphocytes) were excluded from the digital image analysis. Men without a valid telomere measurement were excluded (PCBN lower-grade N=4; PCBN higher-grade N=8).
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Quantifying Telomere Length in Prostate Cancer
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Adenocarcinoma
Cell types
Fluorescence
Fluorescence probe
Fluorescent in situ hybridization
In situ hybridization
Lymphocytes
Microscope
Prostate
Prostate cancer
Telomere
Tissue
Corresponding Organization : Sidney Kimmel Comprehensive Cancer Center
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Protocol cited in 2 other protocols
Variable analysis
independent variables
- None explicitly mentioned
- Telomere length in prostate cancer cells
- Telomere length in prostate CAS cells
- Tissue microarray (TMA) sections containing areas of adenocarcinoma
- Telomere-specific fluorescence in situ hybridization (FISH) probe
- 4',6-diamidino-2-phenylindole (DAPI) for labeling total nuclear DNA
- Exclusion of other cell types (e.g. infiltrating lymphocytes) based on morphological features
- None mentioned
- None mentioned
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