Skeletal Surface Imaging and Aragonite Analysis

To image the surface of whole skeletal fragments, and also for electron back scatter diffraction (EBSD), an FEI Quanta 200F field emission scanning electron microscope (SEM) equipped with a TSL EBSD system was used [28 (link)]. To assess the molecular-scale bonding of the skeleton, a Renishaw inVia Raman spectrometer equipped with a Leica DM 2500 M microscope and 785 nm laser, with the aragonite peak centred at 1085 cm⁻¹ was used [28 (link)]. Full-width half-maximum (FWHM) measurements of the aragonite peak at 1085 cm⁻¹ were collected at the outside tips of newly grown corallites, as close as possible to the sample edge (approx. 5 µm).

Free full text: Click here

Hennige S.J., Wicks L.C., Kamenos N.A., Perna G., Findlay H.S, & Roberts J.M. (2015). Hidden impacts of ocean acidification to live and dead coral framework. Proceedings of the Royal Society B: Biological Sciences, 282(1813), 20150990.

Publication 2015

inVia Raman spectrometer DM 2500 M microscope

Aragonite Electron Microscope Scanning electron microscope Skeleton

Corresponding Organization :

Other organizations : Heriot-Watt University, University of Glasgow, Plymouth Marine Laboratory, University of North Carolina Wilmington, Scottish Association For Marine Science

Top 5 similar protocols

Variable analysis

independent variables

Type of microscope used (FEI Quanta 200F field emission scanning electron microscope (SEM))
Type of EBSD system used (TSL EBSD system)
Type of Raman spectrometer used (Renishaw inVia Raman spectrometer)
Type of microscope used for Raman spectrometry (Leica DM 2500 M microscope)
Wavelength of laser used for Raman spectrometry (785 nm)

dependent variables

Surface features of whole skeletal fragments (imaged using SEM)
Molecular-scale bonding of the skeleton (assessed using Raman spectrometry)
Full-width half-maximum (FWHM) measurements of the aragonite peak at 1085 cm^-1

control variables

Location of FWHM measurements (outside tips of newly grown corallites, as close as possible to the sample edge (approx. 5 µm))

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!