Characterization of Magnetic Nanoparticles

Concerning their physicochemical features, MNPs were analyzing regarding their size, iron content according to previous studies (Friedrich et al. 2021 ; Mühlberger et al. 2019 (link); Stein et al. 2020 ). Their iron content in milligram per milliliter was investigated after diluting them 1:20 in deionized H₂O, liquefying them in 65% nitric acid with atomic emission spectroscopy (AES), using Agilent 4200 MP-AES with an iron solution of 1000 mg Fe/l as an external standard (Bernd Kraft, Duisburg, Germany). MNP particles were diluted to the desired concentration with deionized water for all experiments. Transmission electron microscopy (TEM) images were acquired using the CM30 TEM/STEM (Philips, The Netherlands) operating at 300 kV acquired with a CCD camera, a Tietz Fast Scan-F114 (Tietz Video and Image Processing Systems GmbH, Gauting, Germany). Dispersions at a concentration of 100 µg Fe/ml were dropped onto a carbon-coated copper grids (Plano, Germany) an air-dried at RT.

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Friedrich B., Vogel P., Rückert M.A., Lyer S., Günther J., Wernery U., Joseph S., Müller J., Behr V.C., Alexiou C, & Tietze R. (2023). Detection of viral antibodies in camel sera using magnetic particle spectroscopy. Applied Microbiology and Biotechnology, 107(10), 3329-3339.

Publication 2023

Agilent 4200 MP-AES CM30 TEM/STEM

Acid Carbon Copper Iron Nitric acid Scan Spectroscopy Stem Transmission electron microscopy

Corresponding Organization :

Other organizations : Else Kröner-Fresenius-Stiftung, Universitätsklinikum Erlangen, University of Würzburg, Central Veterinary Research Laboratory

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Variable analysis

independent variables

Dilution of MNPs in deionized H2O (1:20)
Concentration of MNP dispersions (100 µg Fe/ml)

dependent variables

Iron content of MNPs in milligram per milliliter
Size of MNP particles

control variables

Liquefying MNPs in 65% nitric acid
Using atomic emission spectroscopy (AES) with Agilent 4200 MP-AES
Using an iron solution of 1000 mg Fe/l as an external standard
Diluting MNP dispersions to desired concentration with deionized water
Dropping MNP dispersions onto carbon-coated copper grids and air-drying at room temperature
Using transmission electron microscopy (TEM) with CM30 TEM/STEM (Philips, The Netherlands) operating at 300 kV and acquiring images with a CCD camera (Tietz Fast Scan-F114)

positive controls

Iron solution of 1000 mg Fe/l as an external standard

negative controls

Not mentioned

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