Skin Fibroblast and Stem Cell Culture

CRL-2097 human normal skin fibroblasts (HFs) were purchased from the American Type Culture Collection (ATCC: Manassas, VA, USA). Human UCB-MSCs were purchased from the Japanese Collection of Research Bioresources cell bank (JCRB1109, Osaka, Japan) and ATCC (PCS-500-010, Manassas, VA, USA). The HFs were cultured in α-MEM (GIBCO, Carlsbad, CA, USA) fibroblast medium containing 10% fetal bovine serum, GIBCO), 1% minimum essential medium non-essential amino acid (GIBCO), 1% penicillin/streptomycin (GIBCO), and 1% sodium pyruvate (GIBCO). UCB-MSCs were cultured in MesenCult™-ACF Medium (STEMCELL technologies, Cambridge, MA, USA). The hiPSCs reprogrammed from HFs by using Sendai-virus vectors encoding Oct4, Sox2, Klf4, and c-Myc [33 (link)], or episomal vectors (Episomal iPSC reprogramming vectors, Thermo Fisher Scientific, Waltham, MA, USA) [34 (link)], were cultured with mTeSR1 (STEMCELL Technologies) medium.