Preclinical Immunotherapy Combination Protocol

Anti-mouse PD-1 (clone RMP1-14), anti-mouse CTLA-4 (clone 9D9), anti-mouse CD80 (clone 16-10A1), anti-mouse CD86 (clone GL-1), anti-mouse Ly6C (clone Monts 1), anti-mouse CCL2 (clone 2H5), and the isotype-matched IgG controls were purchased from BioXCell. Recombinant murine CCL2 (catalog #250-10), IFN-γ (catalog #315-05), IL4 (catalog #214-14), G M-CSF (catalog #315-03), M-CSF (catalog #315-02) were purchased from PeproTech. Murine lung cancer cell lines 393P, 412P, 344P, 344SQ, and 307P were derived from K-ras^LA1/+p53^R172HΔg/+ mice as previously described in multiple prior lung cancer studies (16 (link)–23 (link)). The LLC-JSP Lewis lung tumor cell line as well as the B16 and B16-OVA melanoma cell lines were maintained in our laboratories (24 (link), 25 ).

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Rodriguez B.L., Chen L., Li Y., Miao S., Peng D.H., Fradette J.J., Diao L., Konen J.M., Alvarez F.R., Solis L.M., Yi X., Padhye A., Gibson L.A., Ochieng J.K., Zhou X., Wang J, & Gibbons D.L. (2023). Targeting immunosuppressive Ly6C+ classical monocytes reverses anti-PD-1/CTLA-4 immunotherapy resistance. Frontiers in Immunology, 14, 1161869.

Publication 2023

Recombinant murine CCL2 IFN-γ IL4 GM-CSF M-CSF

Anti cd80 B16 melanoma Ccl2 Cell lines Clone Ctla 4 Gm csf Ifn γ Isotype Lung Lung cancer M csf Mice Tumor cell line

Corresponding Organization : The University of Texas MD Anderson Cancer Center

Other organizations : Shanghai Jiao Tong University, Shanghai First People's Hospital, The University of Texas Health Science Center at Houston, Bellicum Pharmaceuticals (United States)

Top 5 similar protocols

Variable analysis

independent variables

Anti-mouse PD-1 (clone RMP1-14)
Anti-mouse CTLA-4 (clone 9D9)
Anti-mouse CD80 (clone 16-10A1)
Anti-mouse CD86 (clone GL-1)
Anti-mouse Ly6C (clone Monts 1)
Anti-mouse CCL2 (clone 2H5)
Recombinant murine CCL2 (catalog #250-10)
Recombinant murine IFN-γ (catalog #315-05)
Recombinant murine IL4 (catalog #214-14)
Recombinant murine GM-CSF (catalog #315-03)
Recombinant murine M-CSF (catalog #315-02)

dependent variables

Dependent variables not explicitly mentioned.

control variables

Isotype-matched IgG controls

controls

Positive controls: Isotype-matched IgG controls
Negative controls: Not explicitly mentioned

Annotations

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