Quantitative Real-Time PCR Analysis

For data analyses, a standard curve was constructed by amplification of serially diluted plasmids containing the target sequence. Data were analyzed at the termination of each assay using Sequence Detection Software (Sequence Detection Software, version 1.3.1; Applied Biosystems, Norwalk, CT, USA; Girard et al., 2008 (link), 2010 (link), 2013 (link), 2016 (link); Gonzalez et al., 2013 (link)) as previously described. Data are expressed as the relative quantity of the gene of interest normalized to the relative quantity of the housekeeping gene, ribosomal protein L32.

Free full text: Click here

Guo M., Chang P., Hauke E., Girard B.M., Tooke K., Ojala J., Malley S.M., Hsiang H, & Vizzard M.A. (2018). Expression and Function of Chemokines CXCL9-11 in Micturition Pathways in Cyclophosphamide (CYP)-Induced Cystitis and Somatic Sensitivity in Mice. Frontiers in Systems Neuroscience, 12, 9.

Publication 2018

Sequence Detection Software

Assay Gene Housekeeping gene Plasmids Ribosomal protein l32

Corresponding Organization : University of Vermont

Top 5 similar protocols

Variable analysis

independent variables

Serially diluted plasmids containing the target sequence.

dependent variables

Relative quantity of the gene of interest normalized to the relative quantity of the housekeeping gene, ribosomal protein L32.

control variables

Not explicitly mentioned.

controls

None specified.
None specified.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!