TLR agonists LPS (TLR4), R848 (TLR7/8) and Pam3CSK4 (TLR1/2) (all from Invivogen, San Diego, California, USA) were used at a final concentration of 2 µg/mL, as described previously.77 (link) In addition to these TLR agonists, the following anti-inflammatory drugs were used in this study: IBF and BMS (both from Sigma-Aldrich). IBF was resuspended in sterile PBS, while BMS was initially resuspended in 100% ethanol before diluting 1:5 with sterile PBS, and both drugs were used at a final concentration of 1 µg/mL, which was the drug concentration previously optimized in anti-inflammatory drug titration experiments (data not shown).
Protocol detail
Immune Modulation by TLR Agonists and Anti-inflammatory Drugs
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Corresponding Organization : University of KwaZulu-Natal
Other organizations : Africa Health Research Institute, Max Planck Institute for Infection Biology, University of Manitoba, Columbia University, University of Cape Town
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Variable analysis
independent variables
- LPS (TLR4)
- R848 (TLR7/8)
- Pam3CSK4 (TLR1/2)
- Measured outcomes
- Final concentration of 2 µg/mL for TLR agonists
- Final concentration of 1 µg/mL for IBF and BMS
- Positive controls not explicitly mentioned
- Negative controls not explicitly mentioned
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