Protocol detail

Transcriptional Analysis of R. cellulolyticum

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RNA preparation and reverse transcription were performed from R. cellulolyticum derivative strains grown in minimal medium supplemented with arabinose (2 g L⁻¹) or cellobiose (2 g L⁻¹) as previously described [4 (link)]. For transcriptional analysis, quantitative real-time PCR was performed using SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, Marnes-la-Coquette, France), and the results were analysed with Bio-Rad CFX Manager software, version 3.0 (Bio-Rad, France) as previously described [4 (link)].

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Fosses A., Franche N., Parsiegla G., Denis Y., Maté M., de Philip P., Fierobe H.P, & Perret S. (2023). Role of the Solute-Binding Protein CuaD in the Signaling and Regulating Pathway of Cellobiose and Cellulose Utilization in Ruminiclostridium cellulolyticum. Microorganisms, 11(7), 1732.

Publication 2023

SsoFast EvaGreen Supermix 2X Kit Bio-Rad CFX Manager software, version 3.0

Arabinose Cellobiose Quantitative real time pcr Reverse transcription Strains Transcriptional

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Variable analysis

independent variables

Growth medium supplemented with arabinose (2 g L^-1)
Growth medium supplemented with cellobiose (2 g L^-1)

dependent variables

Transcriptional analysis by quantitative real-time PCR

control variables

R. cellulolyticum derivative strains
Minimal medium

controls

No positive or negative controls were explicitly mentioned.

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