Animals were sacrificed three days after induction of SDs via live decapitation. Fresh ipsilateral frontal cortex, parietal cortex, and hippocampus were dissected, flash frozen on dry ice, and stored at −80°C. Homogenized tissue samples were quantified via BCA assay and immunoblotting was performed. Briefly, protein was loaded into pre-cast gels, run at 200mV and transferred in a semi-dry transfer system. Membranes were incubated in REVERT Total Protein Stain for 5 minutes (LI-COR Biosciences #926–11010), washed, and imaged using Odyssey Imager and ImageStudio software (LI-COR) for later signal normalization to total protein (Aldridge et al. 2008 (link); Eaton et al. 2013 (link); Kirshner and Gibbs 2018 (link)). After incubation in destaining solution (LI-COR #926–11013), blots were then blocked for 1 hour, incubated in appropriate primary antibody overnight at 4°C (mouse anti-GluA1: 1:500, RRID:AB_2315840; mouse anti-GluA2: 1:500, RRID:AB_2232661; mouse anti-GluN2A: 1:250, RRID:AB_2315842; mouse anti-GluN2B: 1:250, RRID:AB_10673405; Antibodies, Inc), then washed, incubated for 1 hour at room temperature in secondary antibody (donkey anti-mouse IgG IRDye 800CW: 1:30,000, RRID:AB_2716622; LI-COR), and imaged with Odyssey Imager. Protein band signals were quantified using ImageStudio (LI-COR) and normalized to total protein stain.