Estimating FP Gene Copy Numbers in A. digitifera

FP gene copy numbers in the A. digitifera genome were estimated by quantitative PCR (qPCR) using 11 A. digitifera specimens. FP gene copy number estimation was performed using previously described methods (Takahashi-Kariyazono et al. 2016 (link)) with minor modifications. Briefly, two new reverse primers (MLWE_qPCR_R1-3 for M/LWE and MiA_CP_e3_R1-3 for CP) were used for qPCR. Primer sequences are shown in supplementary table S2, Supplementary Material online. qPCR was performed using the Thermal Cycler Dice TP800 (Takara). Genomic DNAs of 11 samples (S1601–8, S1610–12) were used as templates.

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Takahashi-Kariyazono S., Sakai K, & Terai Y. (2018). Presence–Absence Polymorphisms of Highly Expressed FP Sequences Contribute to Fluorescent Polymorphisms in Acropora digitifera. Genome Biology and Evolution, 10(7), 1715-1729.

Publication 2018

Thermal Cycler Dice TP800

Dnas Genomic Primer

Corresponding Organization :

Other organizations : The Graduate University for Advanced Studies, SOKENDAI, University of the Ryukyus

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Variable analysis

independent variables

Primer sequences used for qPCR

dependent variables

FP gene copy numbers in the A. digitifera genome

control variables

11 A. digitifera specimens used as templates for qPCR

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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