We performed the analysis of peripheral blood lymphocyte populations by one laser three-color flow cytometry (BD Biosciences FACS Calibur, USA). One-hundred microliter of whole blood was obtained and stained with 20 μl of the monoclonal antibodies (CD3(fluorescein isothiocyanate (FITC)), CD4(FITC), CD8 (peridinin-chlorophyll protein complex (PerCP)), CD16 + 56(APC), and CD19 (phycoerythrin (PE)) (Beckton Dickinson, BD, USA)). Then, the samples were incubated in the dark for 15 min at room temperature.
Karaatmaca B., Cagdas D., Esenboga S., Erman B., Tan C., Turul Ozgur T., Boztug K., van der Burg M., Sanal O, & Tezcan I. (2024). Heterogeneity in RAG1 and RAG2 deficiency: 35 cases from a single-centre. Clinical and Experimental Immunology, 215(2), 160-176.
Other organizations :
Hacettepe University, Sağlık Bilimleri Üniversitesi, Austrian Academy of Sciences, Medical University of Vienna, Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases, St Anna Children's Hospital, CeMM Research Center for Molecular Medicine, Leiden University Medical Center
Monoclonal antibodies (CD3(fluorescein isothiocyanate (FITC)), CD4(FITC), CD8 (peridinin-chlorophyll protein complex (PerCP)), CD16 + 56(APC), and CD19 (phycoerythrin (PE)))
dependent variables
Peripheral blood lymphocyte populations
control variables
Whole blood sample
Incubation time (15 min)
Incubation temperature (room temperature)
Flow cytometry platform (BD Biosciences FACS Calibur, USA)
controls
No positive or negative controls were explicitly mentioned.
Annotations
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