Glutamate Measurement in BMDMs

BMDMs were incubated in phenol red free-medium. The glutamate level was measured after 3 h incubation with auranofin metabolites, using the Amplex^® Red glutamate assay kit (Thermo Fisher Scientific, Waltham, MA, USA). According to the manufacturer’s instruction, the glutamate concentrations of the medium were measured by a fluorescence microplate reader (SpectraMAXi3x, Molecular Devices, Sunnyvale, CA, USA).

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Kim H.Y., Otgontenger U., Kim J.W., Lee Y.J., Kim S.B., Lim S.C., Kim Y.M, & Kang K.W. (2023). Anti-fibrotic effect of aurocyanide, the active metabolite of auranofin. Archives of Pharmacal Research, 46(3), 149-159.

Publication 2023

Amplex® Red glutamate assay kit SpectraMAXi3x

Assay Auranofin Devices Fluorescence Glutamate

Corresponding Organization :

Other organizations : Seoul National University, Hanyang University, Daegu-Gyeongbuk Medical Innovation Foundation, Chosun University

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Variable analysis

independent variables

Auranofin metabolites

dependent variables

Glutamate level

control variables

Phenol red free-medium

controls

No positive or negative controls were explicitly mentioned in the input.

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