Protocol detail

Chitin and flg22 Treatment Assays

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chitin and flg22 treatment assays were performed as described previously [53 (link)]. When the wild-type ZH11 seedings grew to four weeks, the leaf segments that were 2–3 cm in length were collected and balanced in ddH₂O for 12 h, and then they were treated with 1 µM flg22 (synthesized by Sangon Biotech Co., Ltd., Shanghai, China) or 20 µg/mL chitin (Sigma, St. Louis, MO, USA). The samples were collected at 6 h and 12 h after treatment for further analysis.

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Hou H., Fang J., Liang J., Diao Z., Wang W., Yang D., Li S, & Tang D. (2020). OsExo70B1 Positively Regulates Disease Resistance to Magnaporthe oryzae in Rice. International Journal of Molecular Sciences, 21(19), 7049.

Publication 2020

flg22 chitin

After treatment Assays Chitin Cm 2 3 Leaf

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Variable analysis

independent variables

Flg22 treatment
Chitin treatment

dependent variables

Leaf segment response

control variables

Leaf segment size (2-3 cm length)
Leaf segment incubation time (12 h in ddH2O)
Plant age (4 weeks)

controls

Positive control: Not specified
Negative control: Not specified

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