Antibodies, chemicals, and plasmids used in this study are listed in Supplemental Table S1. HEK293T cells (RBC2202; RIKEN BioResource Research Center) were cultured in DMEM with high glucose (Nacalai Tesque) supplemented with 5% fetal bovine serum (FBS). hTERT-RPE1 cells (CRL-4000; American Type Culture Collection) were grown in DMEM/Ham’s F-12 medium (Nacalai Tesque) supplemented with 10% FBS and 0.348% sodium bicarbonate at 37°C in 5% CO2. IFT27-KO cells (cell line #IFT27-2-2) and CEP19-KO cells (cell lines #CEP19-1-2 and #CEP19-1-12) were established from hTERT-RPE1 cells, as described previously (Nishijima et al., 2017 (link); Zhou et al., 2022 (link)).
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Establishment of IFT27 and CEP19 Knockout Cell Lines
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Other organizations : Kyoto University
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Variable analysis
independent variables
- Knockout of IFT27 gene in hTERT-RPE1 cells
- Knockout of CEP19 gene in hTERT-RPE1 cells
- Not explicitly mentioned
- HEK293T cells cultured in DMEM with high glucose supplemented with 5% FBS
- HTERT-RPE1 cells cultured in DMEM/Ham's F-12 medium supplemented with 10% FBS and 0.348% sodium bicarbonate
- Culture conditions: 37°C in 5% CO2
- Positive control: Not mentioned
- Negative control: Not mentioned
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