The DSS (MW: 50 kDa) used in this study was purchased from Shanghai Yuanye BioTechnology Co., Ltd. (Shanghai, China). The broilers in the DSS group were gavaged a DSS solution (1 mL, 2.5% weight/volume) at the same time daily from 21 to 29 days of age. The major reason for the selected treatment schedule was due to the fact that broiler enteric inflammation usually occurs between two to six weeks of age. The volume [19 (link)] and concentration [6 (link)] of the DSS solution used in this study partly refer to the previous two studies.
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