MMP changes were detected in the cells using an MMP assay kit (M8650, Solarbio, Beijing, China) and flow cytometry (19 (link)). The cells were treated with 10, 40, and 70 µM luteolin for 24 h or 48 h. Then, the treated cells were resuspended in a complete media and stained with a JC-1 fluorescence working solution. MMP changes were measured with the FACSCanto II flow cytometer (Becton Dickinson and Company, Franklin Lakes, United States), and the fluorescence intensity was analyzed using the FACSDiva software (version 6.1.3; Becton Dickinson and Company, Franklin Lakes, United States). The relative ratio of red fluorescence to green fluorescence was applied for quantitative analysis of the MMP changes. The data were normalized as fold changes in comparison to the DMSO group. Experiments were repeated in HGC-27 cells (n=12), MFC cells (n=11), and MKN-45 cells (n=4).
Protocol detail
Evaluating Luteolin's Impact on Mitochondrial Membrane Potential
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Corresponding Organization :
Other organizations : Ocean University of China, Binzhou Medical University, Nantong University
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Variable analysis
independent variables
- Luteolin concentration: 10 µM, 40 µM, 70 µM
- Treatment duration: 24 h, 48 h
- Mitochondrial membrane potential (MMP) changes
- Cell lines: HGC-27, MFC, MKN-45
- Positive control: Not mentioned
- Negative control: DMSO group
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