KSHV and EBV viral load assays were performed on peripheral blood mononuclear cells (PBMC) and aliquots of effusions using quantitative real-time polymerase chain reaction (PCR). KSHV and Epstein-Barr virus (EBV) viral DNA (KSHV-VL, EBV-VL) were quantified using primers from KSHV K6 and EBV pol gene regions as previously described.(10 (link)) Cell number quantifications were performed using a human endogenous retrovirus 3 (ERV-3) assay.(24 (link)) PBMC viral loads were expressed as copies per million cell equivalents and effusion viral loads were expressed as copies/106 cells.
Serum and effusion levels of human interleukin [(IL-) IL-2, IL-4, IL-6, IL-1β, IL-8, IL-10, IL-12p70, IL-13)], inducible protein-10 (IP-10), interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and vascular endothelial growth factor (VEGF) were evaluated using the Mesoscale Discovery (MSD) Proinflammatory Panel 1 human kit(25 ). Laboratory results (white cell count, lactate dehydrogenase, protein, glucose) were captured from electronic medical records at time as the effusion and serum samples were collected.
Serum and effusion levels of human interleukin [(IL-) IL-2, IL-4, IL-6, IL-1β, IL-8, IL-10, IL-12p70, IL-13)], inducible protein-10 (IP-10), interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α) and vascular endothelial growth factor (VEGF) were evaluated using the Mesoscale Discovery (MSD) Proinflammatory Panel 1 human kit(25 ). Laboratory results (white cell count, lactate dehydrogenase, protein, glucose) were captured from electronic medical records at time as the effusion and serum samples were collected.
