Cell Cycle Analysis by Flow Cytometry

flow cytometry was performed using the PI single staining method to conduct cell cycle analysis, as described previously (21 (link)). In general, we collected cells and suspended them in 1×PBS and then we fixed the cells in ethanol at 4°C overnight. Next, the cells were centrifuged for 5 min at 2000 rpm and resuspended 1×PBS and were centrifuged again. The cells were mixed with 500 μL of propidium (Beyotime, Shanghai, China) and kept in the dark for 10 min. Then flow cytometry (BD Biosciences, CA, USA) was used to analyze the samples.

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Yang L., Chen X., Qian X., Zhang J., Wu M, & Yu A. (2022). Comprehensive Analysis of the Transcriptome-Wide m6A Methylome in Endometrioid Ovarian Cancer. Frontiers in Oncology, 12, 844613.

Publication 2022

propidium flow cytometry

Cell cycle Cells Ethanol Flow cytometry Propidium Staining method

Corresponding Organization :

Other organizations : Cancer Hospital of Chinese Academy of Medical Sciences, University of Chinese Academy of Sciences, Zhejiang Cancer Hospital

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Variable analysis

independent variables

Cell fixation with ethanol at 4°C overnight

dependent variables

Cell cycle analysis

control variables

Cell collection and suspension in 1×PBS
Cell centrifugation and resuspension in 1×PBS
Incubation with propidium iodide (500 μL) for 10 min in the dark
Flow cytometry analysis

controls

No positive or negative controls were explicitly mentioned in the input text.

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