Purification of Fungal β-Glucan Particles

Fungal β-glucan particles were prepared by treating Saccharomyces cerevisiae zymosan A (Sigma) to remove TLR agonists (depleted zymosan) as previously described (15 (link)). Briefly, zymosan particles were boiled in 10 M NaOH for 30 minutes and then thoroughly washed with PBS and sonicated prior to use. Salmonella minnesota lipopolysaccharide (LPS) was from Invivogen.

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Hassanzadeh-Kiabi N., Yáñez A., Dang I., Martins G.A., Underhill D.M, & Goodridge H.S. (2016). Autocrine type I IFN signaling in dendritic cells stimulated with fungal β-glucans or lipopolysaccharide promotes CD8 T cell activation. Journal of immunology (Baltimore, Md. : 1950), 198(1), 375-382.

Publication 2016

Saccharomyces cerevisiae zymosan A

Agonists Depleted zymosan Lipopolysaccharide Saccharomyces cerevisiae Salmonella Zymosan β glucan

Corresponding Organization : Cedars-Sinai Medical Center

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Variable analysis

independent variables

Treating Saccharomyces cerevisiae zymosan A (Sigma) to remove TLR agonists (depleted zymosan)

dependent variables

Not explicitly mentioned

control variables

Boiling zymosan particles in 10 M NaOH for 30 minutes
Thoroughly washing zymosan particles with PBS
Sonicating zymosan particles prior to use
Using Salmonella minnesota lipopolysaccharide (LPS) from Invivogen

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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