Laser-Induced Choroidal Neovascularization

CNV was generated as previously described [52 (link)]. Briefly, C57BL/6J and Scg3 KO mice were subjected to laser photocoagulation (Argon laser, 532 nm, 100 mW, 0.8 s, 50 μm, 4 spots per retina around the optic disc) on Day 0 (D0) using MICRON IV system (Phoenix). Lesions with choroidal hemorrhage on D0 and linear or fused lesions on D7 were excluded.

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Ji L., Waduge P., Wan W., Tian H., Li J., Zhang J., Chen R, & Li W. (2022). Comparative ligandomics implicates secretogranin III as a disease-restricted angiogenic factor in laser-induced choroidal neovascularization. The FEBS journal, 289(12), 3521-3534.

Publication 2022

MICRON IV system

Argon laser Choroidal hemorrhage Mice Optic disc Photocoagulation Retina Spots

Corresponding Organization : University of Miami

Other organizations : China Medical University, Fourth Affiliated Hospital of China Medical University, First Affiliated Hospital of Zhengzhou University, Everglades University

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Variable analysis

independent variables

Mouse genotype (C57BL/6J and Scg3 KO)

dependent variables

Laser photocoagulation lesion characteristics (choroidal hemorrhage on D0, linear or fused lesions on D7)

control variables

Laser parameters (532 nm, 100 mW, 0.8 s, 50 μm, 4 spots per retina around the optic disc)
Laser system (MICRON IV)

controls

Positive control: None specified
Negative control: None specified

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