Comprehensive Biomarker Assessment Protocol
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Corresponding Organization :
Other organizations : Beijing Friendship Hospital, Capital Medical University, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
Variable analysis
- None explicitly mentioned
- UA1MG level
- Complete blood cell counts
- Lipid panel
- Glycated hemoglobin A1c
- High-sensitivity C-reactive protein (hs-CRP) levels
- Cardiac enzymes involving troponin I (cTnI), creatine kinase myocardial band (CK-MB), and N-terminal pro-B-type natriuretic peptide (NT-proBNP)
- Overnight clean midstream urine, sampled in the morning between 6:00 a.m. and 11:00 a.m., within 24 h of hospital admission
- Sample sent to the Laboratory Medicine Department within 1 h for testing
- Nephelometry assay kit used to test the UA1MG level with the BN II protein analyzer (Siemens, Germany)
- Normal reference range for UA1MG level provided by the manufacturer as 0.0 to 1.2 mg/dL
- Peripheral blood draw sampled immediately on admission
- Complete blood cell counts measured using the fluorescent dyeing flowcytometry method with the XN3000 analyzer (Sysmex, Japan)
- Lipid panel, glycated hemoglobin A1c, and high-sensitivity C-reactive protein (hs-CRP) levels collected on the morning of the second day after at least 8 h of fasting
- Levels of cardiac enzymes involving troponin I (cTnI), creatine kinase myocardial band (CK-MB), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) collected on admission and every morning according to the hospital protocol
- Blood biochemistry parameters and cardiac biomarkers evaluated using test kits with AU5821 biochemistry analyzing equipment (Beckman Coulter Company), according to the manufacturer's instructions
- Positive control: None mentioned
- Negative control: None mentioned
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