Osteoclastogenesis Induction Protocol

Recombinant human colony-stimulating factor 1 (rhCSF1), TNFSF11 (rhTNFSF11) and MCP-1 (rhMCP-1) were purchased from PeproTech, Inc. α-minimal essential medium (α-MEM), penicillin-streptomycin solution, PBS without calcium and magnesium, FBS, DAPI staining solution and Rhodamine-conjugated phalloidin were purchased from Gibco; Thermo Fisher Scientific, Inc. The tartrate-resistant acid phosphatase (TRAP) staining kit, Escherichia coli (E. coli) LPS, and haematoxylin and eosin (H&E) were purchased from Sigma-Aldrich; Merck KGaA. Cell Counting Kit-8 (CCK-8) reagent was obtained from Dojindo Molecular Technologies, Inc. 7ND protein was purified as previously reported (12 (link)).

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Long W., Quan J., Liu Y., Li J., Gong Q, & Jiang H. (2020). 7ND protein exerts inhibitory effects on both osteoclast differentiation in vitro and lipopolysaccharide-induced bone erosion in vivo. Molecular Medicine Reports, 22(1), 97-104.

Publication 2020

MCP-1 (rhMCP-1) penicillin-streptomycin solution FBS Rhodamine-conjugated phalloidin tartrate-resistant acid phosphatase (TRAP) staining kit Escherichia coli (E. coli) LPS haematoxylin and eosin (H&E) Cell Counting Kit-8 (CCK-8) reagent

Calcium Dapi Eosin Escherichia coli Haematoxylin Human colony stimulating factor 1 Magnesium Mcp 1 Penicillin Protein Rhodamine phalloidin Streptomycin Tartrate resistant acid phosphatase Tnfsf11

Corresponding Organization :

Other organizations : Third Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Stomatology Hospital, Southern Medical University

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Variable analysis

independent variables

RhCSF1
RhTNFSF11
RhMCP-1
7ND protein

dependent variables

Not explicitly mentioned

control variables

α-MEM
Penicillin-streptomycin solution
PBS without calcium and magnesium
DAPI staining solution
Rhodamine-conjugated phalloidin
TRAP staining kit
E. coli LPS
Haematoxylin and eosin (H&E)
CCK-8 reagent

positive controls

E. coli LPS

negative controls

Not explicitly mentioned

Annotations

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