Quantitative Analysis of Shikonin Derivatives
Corresponding Organization : Medical University of Warsaw
Other organizations : University of Łódź, Gdańsk Medical University, National and Kapodistrian University of Athens
Protocol cited in 6 other protocols
Variable analysis
- Extraction time (15 min)
- Extraction temperature (40°C)
- Dye fractions extracted from cells and culture media
- Retention times and UV spectra of shikonin and its derivatives (ACS and isobutyrylshikonin)
- Sonication for extraction
- N-hexane as the extraction solvent
- Reduced pressure evaporation of extracts
- Dissolution of dry residue in methanol
- HPLC conditions: gradient elution, flow rate, column, and monitoring wavelengths
- Positive controls: Shikonin, ACS, and isobutyrylshikonin standards
- Negative controls: Not explicitly mentioned
Annotations
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