Scaffold Morphology and Silk Microstructure

The morphology of the scaffold samples was observed with SEM (Hitachi
S-4800, Hitachi, Tokyo, Japan). For SEM, the samples were imaged at 3.0 kV after
sputter-coated with gold.^{21 (link)}The microstructures of silk in aqueous solutions were evaluated with AFM
(Nanoscope V, Veeco, NY, USA). 2 μL of the diluted samples (0.01
wt%) were dropped onto freshly cleaved mica surface and measured by AFM
with a spring constant of 3 N m⁻¹ in tapping mode.

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Xiao L., Zhu C., Ding Z., Liu S., Yao D., Lu Q, & Kaplan D.L. (2018). Growth factor-free salt-leached silk scaffolds for differentiating endothelial cells. Journal of materials chemistry. B, 6(26), 4308-4313.

Publication 2018

S-4800 Nanoscope V

Gold Mica Silk

Corresponding Organization : Suzhou Research Institute

Other organizations : Shenzhen University, Medford Radiology Group, Tufts University

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Variable analysis

independent variables

Imaging technique (SEM and AFM)

dependent variables

Morphology of scaffold samples
Microstructures of silk in aqueous solutions

control variables

Samples were sputter-coated with gold for SEM imaging
Diluted silk samples (0.01 wt%) were used for AFM measurements
AFM measurements were performed in tapping mode with a spring constant of 3 N m^-1

controls

No explicit positive or negative controls were mentioned in the input.

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