Quantification of Sphingolipids by LC-MS

Sphingolipids were extracted and quantified as described previously (23 (link)). Extracted lipids were quantified using the Applied Biosystems 4000 Q-Trap mass spectrometer. The measurements used hexadeuterated S1P as an internal standard and were normalized to total lipid phosphorous determined after wet digestion of total lipid extracts in perchloric acid.

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Jafari N., Drury J., Morris A.J., Onono F.O., Stevens P.D., Gao T., Liu J., Wang C., Lee E.Y., Weiss H.L., Evers B.M, & Zaytseva Y.Y. (2018). De Novo Fatty Acid Synthesis Driven Sphingolipid Metabolism Promotes Metastatic Potential of Colorectal Cancer. Molecular cancer research : MCR, 17(1), 140-152.

Publication 2018

4000 Q-Trap mass spectrometer

Digestion Lipid Perchloric acid Phosphorous Sphingolipids

Corresponding Organization : Markey Cancer Center

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Sphingolipid levels
Total lipid phosphorous

control variables

Hexadeuterated S1P as an internal standard

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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