Mice were sensitized and challenged with OVA as previously described.15 (link) Sensitization was done by subcutaneous injection of OVA (Sigma-Aldrich, St. Louis, MO, USA) adsorbed to 1 mg of aluminum hydroxide (Aldrich, Milwaukee, WI, USA) in 200 μL of normal saline. After sensitization on days 1 and 8, challenges were done by intranasal inhalation of OVA (20 ng/50 μL in phosphate-buffered saline [PBS]) on days 21, 23, 25, and 28 under isoflurane (Vedco, St. Joseph, MO, USA) anesthesia. The control groups were treated in the same way with PBS without OVA. Mice were sacrificed 24 hours after the final OVA challenge after measuring AHR.
Protocol detail
Murine Model of Ovalbumin-Induced Asthma
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Aluminum hydroxide
Anesthesia
Inhalation
Isoflurane
Mice
Normal saline
Phosphate
Saline
Subcutaneous injection
Corresponding Organization :
Other organizations : Seoul St. Mary's Hospital, Catholic University of Korea
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Variable analysis
independent variables
- Sensitization with OVA adsorbed to aluminum hydroxide
- Challenge with intranasal inhalation of OVA
- Airway hyperresponsiveness (AHR)
- Sensitization and challenge with phosphate-buffered saline (PBS) without OVA
- Positive control: Mice sensitized and challenged with OVA
- Negative control: Mice treated with PBS without OVA
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