Hygrosensory behavior was assayed as previously described (Knecht et al., 2016 (link)). Desiccation prior to analysis was performed using a modification of (Lin et al., 2014 (link)). Flies were sorted and placed in tubes containing a strip of filter paper soaked in 3% sucrose and let to dry. The vial stopper was pushed down below the vial lip, ~0.5 g Drierite spread over it, and Parafilm was placed over the top to seal the vial. Vials were kept in an incubator (25°C, 70% RH) for 6 hr before hygrosensory behavior was assessed as described above. Humidity preference data in Figure 3 did not conform to normal distributions (assessed by Shapiro-Wilk test, p<0.01) and were analyzed by Steel with control test using JMP11 (SAS). Humidity preference data in Figure 4 did conform to normal distributions and were analyzed using Tukey HSD using JMP11 (SAS).
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