Measuring 2,3-Linked Sialic Acid on Cell Surface

For measuring the level of 2,3-linked sialic acid on the cell surface, cells were detached from plates using trypsin and stained with fluorescein-labeled MALI (Vector Laboratories). As a control, HeLa cells were treated with 100 milliunits/ml neuraminidase from Clostridium perfringens (Sigma-Aldrich) as described previously (10 (link)). Flow cytometry was performed using a BD Accuri flow cytometer (BD Biosciences), and the data were analyzed using the FlowJo program.

Free full text: Click here

Kim H.S., Lee K., Bae S., Park J., Lee C.K., Kim M., Kim E., Kim M., Kim S., Kim C, & Kim J.S. (2017). CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection. The Journal of Biological Chemistry, 292(25), 10664-10671.

Publication 2017

MALI neuraminidase from Clostridium perfringens BD Accuri flow cytometer

Cells Clostridium perfringens Flow cytometry Hela cells Neuraminidase Sialic acid Trypsin Vector

Corresponding Organization : Korea Research Institute of Chemical Technology

Other organizations : Hanyang University

Top 5 similar protocols

Variable analysis

independent variables

Treatment with 100 milliunits/ml neuraminidase from Clostridium perfringens

dependent variables

Level of 2,3-linked sialic acid on the cell surface

control variables

Detachment of cells from plates using trypsin
Staining of cells with fluorescein-labeled MALI (Vector Laboratories)
Flow cytometry using a BD Accuri flow cytometer (BD Biosciences)
Data analysis using the FlowJo program

controls

Positive control: HeLa cells treated with 100 milliunits/ml neuraminidase from Clostridium perfringens

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!