Murine Melanoma Cell Line Protocols

The murine melanoma cell line SM1 (a BRAF^V600E-driven melanoma) was obtained from the laboratory of Dr. A. Ribas at the University of California Los Angeles [36 (link)]. The murine melanoma cell line B16F10 was purchased from ATCC (Manassas, VA, USA). Cell culture media Dulbecco’s modified Eagle Medium (DMEM) was purchased from Thermo Fisher Scientific and Hyclone RPMI 1640 media was purchased from GE Healthcare Life Sciences (Pittsburgh, PA, USA). When needed, cell culture media was supplemented with 1% penicillin/streptomycin (PenStrep) purchased from Corning (Corning, NY, USA). Fetal bovine serum (FBS) was obtained from Thermo Fisher Scientific.

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Ledezma D.K., Balakrishnan P.B., Cano-Mejia J., Sweeney E.E., Hadley M., Bollard C.M., Villagra A, & Fernandes R. (2020). Indocyanine Green-Nexturastat A-PLGA Nanoparticles Combine Photothermal and Epigenetic Therapy for Melanoma. Nanomaterials, 10(1), 161.

Publication 2020

Dulbecco’s modified Eagle Medium (DMEM) Hyclone RPMI 1640 media penicillin/streptomycin (PenStrep) Fetal bovine serum (FBS)

Cell Cell culture Cell line Culture media Eagle Fetal bovine serum Melanoma Murine Penicillin Ribas Streptomycin

Corresponding Organization : George Washington University

Other organizations : Children's National

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Variable analysis

independent variables

Murine melanoma cell line (SM1 and B16F10)

dependent variables

Not explicitly mentioned

control variables

Cell culture media (DMEM and RPMI 1640)
Penicillin/streptomycin (PenStrep) supplement
Fetal bovine serum (FBS)

controls

Positive control: Not specified
Negative control: Not specified

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