Microfluidic Invasion and Metastasis Assay

Previously developed invasion and metastasis microfluidic devices [41 (link), 42 (link)] were employed. A monolayer-invasion microfluidic chip and a multilayer-metastasis microfluidic chip were constructed of poly-dimethylsiloxane (PDMS, Silgard 184, Dow Chemical), which was replicated from SU-8 3035 negative photoresist (Microchem Corp.)-patterned wafers. Cell-inlet holes and culture chambers were created using differently sized punchers (1.5 mm and 3 mm diameter). After the PDMS chips were prepared, they were bonded irreversibly to a glass substrate after oxygen-plasma treatment for 90 s. Before use, the device was sterilized with UV light for 30 min.

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Wang L., Wang J., Yin X., Guan X., Li Y., Xin C, & Liu J. (2022). GIPC2 interacts with Fzd7 to promote prostate cancer metastasis by activating WNT signaling. Oncogene, 41(18), 2609-2623.

Publication 2022

Silgard 184 SU-8 3035 negative photoresist

Cell Chips Device Metastasis Microfluidic devices Oxygen treatment Plasma Poly dimethylsiloxane Uv light

Corresponding Organization :

Other organizations : Dalian Medical University, First Affiliated Hospital of Dalian Medical University

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Variable analysis

independent variables

Microfluidic device type: monolayer-invasion microfluidic chip or multilayer-metastasis microfluidic chip

dependent variables

Invasion and metastasis outcomes

control variables

PDMS (Silgard 184, Dow Chemical) as the material for chip construction
SU-8 3035 negative photoresist (Microchem Corp.) for patterning the wafers
Cell-inlet holes and culture chambers created using differently sized punchers (1.5 mm and 3 mm diameter)
Irreversible bonding of PDMS chips to a glass substrate after oxygen-plasma treatment for 90 s
Sterilization of the device with UV light for 30 min before use

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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