Tetrazolium Chloride Assay for Dehydrogenase Activity
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Corresponding Organization :
Other organizations : Chaudhary Charan Singh Haryana Agricultural University
Variable analysis
- Treatments applied to the seeds
- Dehydrogenase activity of the seeds, expressed as optical density
- Number of seeds used per treatment (25)
- Preconditioning duration (6 hours)
- Incubation in Tetrazolium Chloride solution (5 ml of 0.1% solution, 2 hours at 40°C)
- Washing and drying of embryos
- Elution of Formazan in methyl cellosolve (5 ml, overnight)
- Optical density measurement at 470 nm using a Spectrophotometer
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